RNA purification is of critical importance in the preparation of samples for use in several applications including cDNA library construction, gene expression assay, in vitro translation, microarrays, Northern blot analysis, nuclease protection assays, real-time quantitative PCR, reverse transcription PCR, RNA mapping etc. Low quality RNA may strongly compromise the experimental results of these applications which are often labor-intensive, time-consuming, and highly expensive.
However, RNA purification faces lot of challenges such as extracting high quality RNA from different types of cells and tissues, extracting RNA from scarce samples, especially in clinical settings. The most common method of RNA purification is phenol extraction. Even most of the kits currently available use a preliminary phenol extraction before further purification steps. However, the major drawback of phenol extraction is that, the RNA is contaminated with significant amounts of both DNA and polysaccharides as both can be present in the aqueous layer after phenol extraction. Both contaminants are precipitated along with RNA using ethanol or isopropanol and depending on the subsequent use of the RNA, they may affect further analyses.1 Moreover, phenol extraction is also cumbersome and hazardous to use.
Bio-Synthesis Inc. has launched the RNATotalTM Purification kit which can alleviate all challenges faced by researchers in obtaining quality RNA purification at unbelievably low price.
This kit uses a proprietary resin-based technology. The kits are available in both columns as well as 96-well format.
- RNATotal TM does not require phenol extraction step.
- It can extract total RNA including large messenger RNA (mRNA), small interfering (siRNA) RNA and microRNA from all samples.
- It can extract RNA from as little as a single cell and does not require a carrier to extract RNA.
- It can extract total RNA from all kinds of specimens including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, plants and viruses.
Besides RNATotalTM Purification kit, Bio-Synthesis also has several other sample purification kits that are suited for different applications. There is also a composite kit for RNA, DNA and protein purification.