Bio-Synthesis offers Alexa 514 fluorescent dye oligonucleotide labeling services. Similar to Alexa 488, Alexa 514 is a mix isomer green-fluorescent dye has superior brightness and photostability, and it can be detected using standard fluorescein, Oregon Green dye or Alexa Fluore 488 dye filter sets. By using instruments that collect spectral distribution information, such as the Zeiss LSM 510 META and the Leica TCS SP2, the signal from the Alexa Fluor 514 dye can be distinguished from both the Alexa Fluor 488 and Alexa Fluor 500 dyes.

The fluorescence conjugates has peak excitation wavelength in 517 nm and emission wavelength in 542.

  • Designed to be optically resolved from the Alexa Fluor 488 dye using spectral imaging instruments with linear-unmixing software
  • Optimal dye for the 514 nm spectral line of the argon-ion laser
  • Exitation in 517 nm
  • Emission in 542 nm

Alexa 514 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for Alexa 514 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

Alexa 514 Oligo Labeling


Fluorescent dye Label

Modification Code:


Chemical Formula:


Exact Mass:


Formula Weight:


Excitation (nm):

517 nm

Emission (nm):

542 nm


Bio-Synthesis Inc. Oligo Structure


Dual HPLC required

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.