Bio-Synthesis offers Alexa 532 fluorescent dye oligonucleotide labeling services. Alexa 532 is a yellow fluorescent dye with excitation suitable for the frequency-doubled Nd:YAG laser line. This dye is pH-insensitive over a wide molar range which permit stable signals in live-cell imaging application.
Alexa 532 conjugates has excitation maximum at 532 nm and emission maximum at 554 nm which is puarticulary useful in multicoloar applications, including super-resolution microscopy (SRM) applications, including STORM, SIM, STED and TPE.
Alexa 532 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 532 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.