Bio-Synthesis offers Alexa  532 fluorescent dye oligonucleotide labeling services. Alexa 532  is a yellow fluorescent dye with excitation suitable for the frequency-doubled Nd:YAG laser line. This dye is pH-insensitive over a wide molar range which permit stable signals in live-cell imaging application. 

Alexa 532 conjugates has excitation maximum at 532 nm and emission maximum at 554 nm which is puarticulary useful in multicoloar applications, including super-resolution microscopy (SRM) applications, including STORM, SIM, STED and TPE.

  • Bright and photostable dye with spectra intermediate between those of fluorescein and tetramethylrhodamine
  • Fluorescence output unmatched by any other spectrally similar dye
  • pH-insensitive fluorescence between pH 4 and 10
  • Ideal for excitation by the 532 nm frequency-doubled principal line output of the Nd:YAG laser

Alexa 532 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for Alexa 532 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

Alexa 532 Oligo Labeling


Fluorescent dye Label, Yellow Fluorescent Dye

Modification Code:


Chemical Formula:


Exact Mass:


Formula Weight:


Excitation (nm):

532 nm

Emission (nm):

554 nm

Extinction Coefficient, ε(λ):



Dual HPLC required

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.