Bio-Synthesis offers Alexa 660 fluorescent dye oligonucleotide labeling services. Alexa 660 has long wavelength emiision with optimal excited by the 647 nm spectral line of the krypton-ion laser and well excited by the 633 nm spectral line of the He-Ne laser. Alexa 660 dye can be use as a second label for use with allophycocyanin (APC) conjugates in flow cytometry applications.
Alexa 660 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 660 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.