Bio-Synthesis offers ATTO 488 fluorescent dye oligonucleotide labeling services. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and very little triplet formation. Thus ATTO 488 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.

ATTO 488 is a fluorescent label with excellent water solubility. Its fluorescence is excited most efficiently in the range 480-515 nm.  For instance the 488 nm line of the Argon-Ion laser is very suitable for excitation. ATTO 488 offers an alternative for Alexa 488, FITC, FAM.


  • Replacement dye for Alexa488, FITC, FAM
  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent water solubility
  • Very little aggregation
  • Net charge of -1
ATTO488 Wavelength

ATTO 4488 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 488 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

ATTO 488 Fluorescent Dye Oligonucleotide Labeling


Fluorescent dye Label, FITC, Alexa Fluor 488 Alternative

Modification Code:


Absorption λmax:

501 nm

Emission (nm):

523 nm

Extinction Coefficient, ε(λ):


Fluorescence Maximum, λfl:

523 nm

Fluorescence QuantumYield, η:



Bio-Synthesis Inc. Oligo Structure


dual HPLC

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.