Bio-Synthesis offers ATTO 495 fluorescent dye oligonucleotide labeling services. ATTO 495 is a fluorescent label derived from the well-known dye Acridine Orange. ATTO 495 is moderately hydrophilic. Its fluorescence can be excited efficiently in the range 465-510 nm. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye shows intense and long-lived phosphorescence in solid matrix or at low temperatures.

ATTO 495 wavelength

ATTO 495 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 495 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

ATTO 495 Fluorescent Dye Oligonucleotide Labeling


Fluorescent dye Label

Modification Code:


Absorption λmax:

495 nm

Excitation (nm):

527 nm

Extinction Coefficient, ε(λ):



Bio-Synthesis Inc. Oligo Structure


dual HPLC

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.