Bio-Synthesis offers ATTO 514 fluorescent dye oligonucleotide labeling at any position of an oligonucleotide. ATTO 514 can be excited efficiently in the range 510-535 nm. A suitable source of excitation is the 514 nm line of an Argon-Ion laser. ATTO 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield, and exceptional thermal and photo-stability. Thus ATTO 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.
ATTO 514 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for ATTO 514 fluorescent dye oligonucleotide labeling services.
Product Name:
Category:
Modification Code:
Absorption λmax:
Emission (nm):
Extinction Coefficient, ε(λ):
Structure:
Purification:
Delivery Format:
Shipping Conditions:
Storage Conditions:
-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.