Bio-Synthesis offers ATTO 520 fluorescent dye oligonucleotide labeling services. ATTO 520 is related to Rhodamine 6G.  Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. 

The fluorescence of ATTO 520 is excited most efficiently in the range 510-535 nm,  A suitable source of excitation is an Argon-Ion laser using the 514 nm line. They dye can be use as an alternative dye for JOE, TET dye.


  • Replacement dye for JOE, TET
  • High fluorescence yield
  • High thermal and photo-stability
  • Moderate hydrophilic
  • at pH >7 reversible formatio of colorless pseudobase
  • Cationic dye closely related to well-known Rhodamine 6G

ATTO520 Wavelength

ATTO 520 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO520 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

ATTO 520 Fluorescent Dye Oligonucleotide Labeling


Fluorescent dye Label

Modification Code:


Absorption λmax:

516 nm

Excitation (nm):


Emission (nm):

538 nm

Extinction Coefficient, ε(λ):



Bio-Synthesis Inc. Oligo Structure


dual HPLC

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.