Bio-Synthesis offers ATTO 532 fluorescent dye oligonucleotide labeling at any position of an oligonucleotide. ATTO 532 is a fluorescent label related to the well-known dye Rhodamine 6G. This dye can be excited efficiently in the range 515-545 nm. A suitable source of excitation is the 532 nm output of the frequency-doubled Nd:YAG laser.

Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and excellent water solubility. Thus ATTO 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.


  • Replacement dye for Alexa 532, HEX
  • High Fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent water solubility
  • Very little aggregation
  • Net Charge of -1

ATTO532 Wavelength

ATTO 532 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO532 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

ATTO 532 Fluorescent Dye Oligonucleotide Labeling


Fluorescent dye Label

Modification Code:


Absorption λmax:

532 nm

Emission (nm):

553 nm

Extinction Coefficient, ε(λ):



Bio-Synthesis Inc. Oligo Structure


dual HPLC

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.