ATTO 542 Fluorescent Dye Oligonucleotide Labeling

Bio-Synthesis offers ATTO 542 fluorescent dye oligonucleotide labeling services. ATTO 542 has out standing flourescence intensity, it can be used to replace, Cy3 and Cy3B. It can be excited efficiently in the range 520-555 nm.

ATTO 542  Replacement Dye

Dye	ABS [nm]	EM [nm]
ATTO 542	542	557
Cy3	550	562
Cy3B	559	570

Characteristic features of the label are strong absorption and high photostability. The dye shows a very high fluorescence quantum yield even after coupling to a bio-molecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.

ATTO 542 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO542 fluorescent dye oligonucleotide labeling services.