Bio-Synthesis offers ATTO550 fluorescent dye oligonucleotide labeling services. ATTO 550 is a fluorescent label related to Rhodamine 6G and Rhodamine B.  Characteristic features of the label are strong absorption, high fluorescence quantum yield, and has high thermal and photo-stability.

ATTO 550 consists of three isomers with practically identical absorption and fluorescence. The fluorescence is excited most efficiently in the range 540 - 565 nm. They dye can be use as an alternative dye for Alexa flour 555 or NED fluorescent dye. 


  • Replacement dye for TAMRA, CY3
  • High Fluorescence yield
  • High thermal and photo-stability
  • Moderate hydrophilic
  • Cationic dye
  • Mixture of three diastereomers
ATTO 550 Wavelength

ATTO 550 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 550 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

ATTO 550 Fluorescent Dye Oligonucleotide Labeling


Fluorescent dye Label, Alexa Fluor 555, NED alternative dye

Modification Code:


Absorption λmax:


Emission (nm):


Extinction Coefficient, ε(λ):



dual HPLC

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.