Bio-Synthesis offers ATTO565 fluorescent dye oligonucleotide labeling services. ATTO 565is a fluorescent label related to the class of Rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and has high thermal and photo-stability. ATTO 565 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.

ATTO 565 consists of two isomers with practically identical absorption and fluorescence. The fluorescence is excited most efficiently in the range 545- 575nm. They dye can be use as an alternative dye for  PET fluorescent dye


  • Replacement dye for Cy3.5, ROX, PET
  • Excitation range: 545-575 nm
  • High fluorescence yield
  • High thermal and photo-stability
  • Cationic dye
  • Supplied as mxiture of two isoemer (single isomer available)
ATTO565 Wavelength

ATTO 565 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

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for ATTO 565 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

ATTO 565 Fluorescent Dye Oligonucleotide Labeling


Fluorescent dye Label, PET Alternative Dye

Absorption λmax:


Extinction Coefficient, ε(λ):


Fluorescence Maximum, λfl:



Bio-Synthesis Inc. Oligo Structure


dual HPLC

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.