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Bio-Synthesis offers ATTO 633 fluorescent dye oligonucleotide labeling services. ATTO 633 is a red specral dye and has characteristic features of the label are strong absorption, high fluorescence quantum yield, and has high thermal and photo-stability. ATTO 633 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.

ATTO 633 is a cationic dye, it excited most efficiently in the range 610-645 nm which most suitable for excitation source using He:Ne laser or a diode-laser emitting at 635 nm.  They dye can be use as an alternative dye for LIZ dye, and Alexa 633.

  • Replacement dye for LIZ or Alexa633
  • High fluorescence yield
  • High thermal and photo-stability
  • Moderate hydrophilic
  • Cationic dye
ATTO633 Wavelength ATTO 633 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 633 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

ATTO 633 Fluorescent Dye Oligonucleotide Labeling

Category:

Fluorescent dye Label, LIZ Dye Alternative

Absorption λmax:

629

Excitation (nm):

657

Extinction Coefficient, ε(λ):

130,000

Fluorescence QuantumYield, η:

64

Fluorescence Lifetime, τ:

3.2

Purification:

HPLC or PAGE

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.