Bio-Synthesis offers many type of biotin oligonucleotide modification synthesis services. Biotinylated oligonucleotide are been used in a variety of biology assays and affinity purification applications. Biotinylated oligos are most commonly used as probes or primers in a variety of in vitro and in vivo applications. These modified oligos has high affinity for streptavidin, and can be found to a streptavidin matrix and used for either column or spin chromatography to purified DNA binding protein. The streptavidin-biotin-oligonucleotide complex is incubated with a crude cell extract containing nuclear proteins. Following appropriate washes, the proteins that bind selectively to the oligonucleotide sequence can be eluted under conditions that disrupt the protein:DNA complex. Because the binding of biotin to streptavidin is essentially irreversible and is resistant to chaotropic agents and extremes of pH and ionic strength, the elution conditions can be relatively stringent.
Biotin can be added to the 5’- or 3’-ends of an oligo using either a C6 (standard) or TEG (tetra-ethyleneglycol, 15 atom) spacer arm. 5' Biotin-TEG requires purification. Internal biotin modification can be introduced using a biotin dT base, which also requires additional purification. Contact us for biotin modified oligonucleotide synthesis services.
Biotin Oligonucleotide Modification
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
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