Cascade Blue Fluor Dye, Blue Emission
Cascade Blue™ is a highly fluorescent blue-emitting dye designed for applications requiring excitation with UV or violet light sources. The fluorophore exhibits a relatively large Stokes shift, high fluorescence intensity, and good photostability, making it well suited for sensitive fluorescence detection and multiplex imaging experiments.
When conjugated to DNA, RNA, PNA, aptamers, or other synthetic oligonucleotides, Cascade Blue™ provides a compact fluorescent label that minimally perturbs nucleic acid hybridization while enabling sensitive optical detection. Because its emission is well separated from green, orange, and far-red fluorophores, Cascade Blue™ is frequently selected as a FRET donor or as one component of multicolor fluorescence assays.
Bio-Synthesis offers custom Cascade Blue™-modified oligonucleotides for DNA, RNA, PNA, LNA/BNA, aptamers, molecular beacons, siRNA, antisense oligonucleotides, CRISPR guide RNAs, and diagnostic probes. The dye may be incorporated at the 5′ terminus, 3′ terminus, or internal positions, depending on the synthesis strategy.
Typical applications include:
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Hybridization probes
- Molecular beacons
- FRET donor labeling
- Biosensor development
- DNA nanotechnology
- Multiplex fluorescence assays
- Molecular diagnostics
Typical Specifications
| Property |
Specification |
| Modification |
Cascade Blue™ |
| Modification Type |
Fluorescent dye |
| Synthesis Chemistry |
Phosphoramidite (direct synthesis) or NHS ester (post-synthetic conjugation), depending on the labeling strategy |
| Dye Family |
Cascade Blue™ fluorophore |
| Spacer |
C3, C6, or linker-dependent spacer (product dependent) |
| Excitation Maximum |
Approximately 400 nm |
| Excitation Color |
Violet |
| Emission Maximum |
Approximately 420 nm |
| Emission Color |
Blue |
| Recommended Laser |
405 nm |
| Typical Placement |
5′ end, 3′ end, or internal positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, LNA/BNA, siRNA, ASOs, aptamers, molecular probes |
| Typical Applications |
Fluorescence microscopy, flow cytometry, FRET donor labeling, hybridization assays, biosensors, multiplex fluorescence imaging |
| Comparable Dyes |
Alexa Fluor® 350, ATTO® 390, DY-350XL, AMCA (approximate spectral equivalents) |
Product Information
Cascade Blue Fluor Dye, Blue Emission
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Haugland RP. The Handbook: A Guide to Fluorescent Probes and Labeling Technologies. Molecular Probes (Thermo Fisher Scientific).
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
- Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155.
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