Bio-Synthesis offer Cy3 fluorescent dye oligonucleotide labeling. Cy3, tetramethylrhodamine (TRITC) is a  bright, orangefluorescent dye that can be excited by mercury arc lamps using the 532 nm laser line and detected with TRITC (tetramethylrhodamine) filter sets. Cy3 labeled probe can be use for visual color application such as imaging,  flow cytometry, and genomic applicatiosn.  It is also the basis for the classic lipophilic tracer DiI and its variants.

The fluorescence conjugates has peak excitation wavelength in 554 nm and emission wavelength in 568 nm and the bright orange fluorescence can be use to as alternate dye for Alexa 555.   

Cy3  dye can be incorporated at any position within an Oligonucleotide  at time of synthesis or post-synthetically. An oligonucleotide modified with a primary amine group is used to covalently attach dye with oligonucleotide via NHS ester chemistry; other cross-linking chemistries are also available depending on particular project specifications . Dual HPLC purification is highly recommended when labeling post-synthetically.

Contact us for CY3 fluorescent dye oligonucleotide labeling services.

Product Information


Product Name:

Cy3 Dye Oligo Labeling

Alternate Name:

Tetramethylrhodamine (TRITC)


Fluorescent dye Label, Alexa 555 alternative

Modification Code:


Chemical Formula:


Exact Mass:


Formula Weight:


Excitation (nm):


Emission (nm):



Bio-Synthesis Inc. Oligo Structure



Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.