Cy3B Fluor Dye, Orange Emission

Cy3B™ NHS Ester is an advanced cyanine fluorophore engineered to provide significantly improved photostability and brightness compared with traditional Cy3 dyes. The dye contains an N-hydroxysuccinimide (NHS) ester reactive group that selectively reacts with primary amines, enabling efficient post-synthetic labeling of amino-modified oligonucleotides.

Following conjugation, Cy3B-labeled DNA, RNA, PNA, aptamers, antisense oligonucleotides, and molecular probes exhibit intense orange fluorescence with excellent resistance to photobleaching. These properties make Cy3B particularly valuable for demanding fluorescence applications such as confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, super-resolution imaging, and single-molecule FRET (smFRET) experiments.

Bio-Synthesis offers custom Cy3B NHS ester-labeled oligonucleotides by conjugating Cy3B to amino-modified oligonucleotides at the 5′ end, 3′ end, or internal amino-modified positions.

Typical applications include:

• Fluorescence microscopy
• Confocal microscopy
• Single-molecule fluorescence
• smFRET
• Flow cytometry
• Hybridization probes
• Molecular beacons
• DNA nanotechnology
• Biosensor development
• Live-cell imaging
• Multiplex fluorescence assays

Typical Specifications

Product Information

 

Product Name:

Cy3B Fluor Dye, Orange Emission

Category:

Orange fluorescent dye

Modification Code:

[Cy3B-NHS]

Absorption λmax:

559 nm

Excitation (nm):

559

            

Emission (nm):

571

            

Extinction Coefficient, ε(λ):

130,000

Structure:

Purification:

Dual HPLC required

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. Cooper M, Ebner A, Briggs M, et al. Cy3B™: improving the performance of cyanine dyes. Journal of Fluorescence. 2004;14(2):145-150.
2. Roy R, Hohng S, Ha T. A practical guide to single-molecule FRET. Nature Methods. 2008;5(6):507-516.
3. Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
4. Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.

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