NPOM-caged dT

NPOM-caged dT, is a nitrophenyl-oxycarbonyl photoactivated group that was designed to inhibit or mask base-pair formation which prevents regular hybridization for the study of antisense regulation of gene expression, light regulated PCR, site directed mutagenesis, study of neurotransmition, celluar signaling, enzyme activity and ion channels and transporters.

The attachment of a photolabile protecting group, 6-nitropiperonyloxymethyl (NPOM), can be removed by UV light at 365nm. This modified base permits activation of biologicaly active molecules at specific locations and effectively renders the molecule inactive. This is refered to as "caging". After irradiation by UV light at specific wavelength, the protecting group is removed, the biological properties of the ‘uncaged’ molecule are restored and cause no DNA damage.

As a general design rule, oligonucleotides containing NPOM-caged dT at every five or six bases do not hybridize to their complementary strand. However, fewer additions may be tolerated and efficient hybridization was still observed when only a single NPOM-caged dT residue was included.

Contact us for light-activated NPOM-caged oligonucleotide synthesis.

Product Information

 

Product Name:

NPOM-caged dT

Chemical Name:

NPOM-dT

Category:

caged nucelobase; photocativation

Modification Code:

[NPOM-dT]

Structure:

Purification:

HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. D.D. Young, W.F. Edwards, H. Lusic, M.O. Lively, and A. Deiters, Chem Commun, 2008, 462-4.
2. D.D. Young, H. Lusic, M.O. Lively, J.A. Yoder, and A. Deiters, ChemBioChem, 2008, 9, 2937-40.
3. D.D. Young, J.M. Govan, M.O. Lively, and A. Deiters, ChemBioChem, 2009, 10, 1612-6.
4. D.D. Young, H. Lusic, M.O. Lively, and A. Deiters, Nucleic Acids Res, 2009, 37, e58.
5. D.D. Young, M.O. Lively, and A. Deiters, J Am Chem Soc, 2010, 132, 6183-93.
6. J.M. Govan, M.O. Lively, and A. Deiters, J Am Chem Soc, 2011, 133, 13176-82.

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