N1-Methyl-deoxyadenosine, N1-Me-dA Oligonucleotide Modification
Bio-Synthesis offers N1-Methyl-deoxyadenosine (N1-Me-dA)modified base oligonucleotide synthesis and modificaiton. The methylation at N1 position of the nucleoside is primarily used in the study of DNA damage and repair mechanisms related to alkylation damage in the environment or inside cells. Such agents introduce various and numerous lesions in DNA and some are even carcinogenic in mammals. To counteract the effects of these lesions, most organisms express several mechanisms for repairing alkylation damage in DNA. The N1-Me-dA lesion is primarily generated by SN2 alkylating reagents such as methyl methanesulfonate and dimethylsulfate, which react with the N1 position of adenine1. In cells, N1-methyl-dA acts as a lethal DNA replication block, but is not very mutagenic (1% A to T transversion in E. coli), and is repaired by the enzyme AlkB by direct reversal2,3. Because the N1 position of adenine is involved in hydrogen bonding of A : T Watson-Crick base pairing, methylation of this site was expected to disrupt hydrogen bonding. However, NMR analysis revealed that N1-methylation actually alters the A:T base-pairing interactions from Watson-Crick to (syn)N1-methyl-A : (anti)T Hoogsteen, thus providing insight into why AlkB repair of N1-Methyl-dA lesions is 10X more efficient on ssDNA over dsDNA4. The avialability of N1-Me-dA enable researchers to obtain oligonucleotide substrate or prototype for mutagenic studies.
Contact Bio-Synthesis for modified N1-Methyl dA oligonucleotide synthesis.
Product Information
N1-Methyl-deoxyadenosine, N1-Me-dA Oligonucleotide Modification
RNA minor base, Structural Studies, DNA damage and repair, Mutagenesis
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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