The ability to conjugate selectively through chemical modification is vital to biomedical research.  Nitrilotriacetic acid (NTA) is a chelating agent that forms a coordination complex with various metal ions including Co2+ and Cu2+.  The capacity of modified NTA to coordinate with Ni2+, allowing it to bind to His-tag, opened up opportunity in multiple biological applications (replacing the previously used iminodiacetic acid).  It greatly simplified the purification process of recombinant proteins with His-tag expressed in prokaryotic or other contexts (Li et al., 2012).  Using nanoparticles with immobilized NTAs, selective purification of His-tagged proteins was achieved (Kim et al., 2007).  To observe specific proteins in living cells, a membrane-permeable NTA-based fluorescent probe was developed that labeled His-tagged proteins in vivo to allow real-time visualization (Lai et al., 2015).  In the arena of DNA nanotechnology, which employs nucleic acids to self-assemble nanoparticles (Robert, 2011; Rothemund et al., 2006), His-tagged recombinant proteins were linked by functionalizing DNA with NTA groups (Goodman et al 2009).  For the diagnosis of thrombin, a microplate with immobilized DNA aptamers was developed, which was terminally hybridized to a NTA-oligonucleotide linked to a His-tagged reporter; upon binding of thrombin to the aptamer, the reporter is released, dampening the signals generated (Shimada et al, 2012).  Thus, NTA-modified oligonucleotides can be used for a wide range of applications.

Nitrilotriacetate (NTA) modified oligonucleotide has high affinity to His-tag on recombinant protein via the complexation of Ni2+. NTA modified oligo can be achieve by post-synthetic conjugation using a thiol functional group, dual HPLC purification is require. This modificaiton can be place at 5', 3' and internally using a DTSPA thiol modified base.

Product Information


Product Name:

Nitrilotriacetate (NTA) Oligo Conjugate

Modification Code:



Bio-Synthesis Inc. Oligo Structure


dual HPLC

Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.


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Kim JS, Valencia CA, Liu R, Lin W.  Highly-efficient purification of native polyhistidine-tagged proteins by multivalent NTA-modified magnetic nanoparticles. (2007).  Bioconjug Chem. 18:333-41.  PMID: 17311440


Lai YT, Chang YY, Hu L, Yang Y, Chao A, Du ZY, Tanner JA, Chye ML, Qian C, Ng KM, Li H, Sun H.  Rapid labeling of intracellular His-tagged proteins in living cells.  (2015).  Proc Natl Acad Sci USA  112:2948-53. PMID: 25713372  doi: 10.1073/pnas.1419598112.


Li S, Clémençon B, Catty P, Brandolin G, Schlattner U, Rousseau D.  Yeast-based production and purification of HIS-tagged human ATAD3A, A specific target of S100B. (2012). Protein Expr Purif.  83:211-6.  PMID: 22542587  doi: 10.1016/j.pep.2012.04.005.


Robert F. DNA nanotechnology grows up. (2011). Science. 332: 1140–1143. PMID 21636754.  doi:10.1126/science.332.6034.1140.


Rothemund PWK.  Folding DNA to create nanoscale shapes and patterns.  (2006).  Nature. 440: 297–302. PMID 16541064.  doi:10.1038/nature04586.


Shimada J, Maruyama T, Kitaoka M, Kamiya N, Goto M.  Microplate assay for aptamer-based thrombin detection using a DNA-enzyme conjugate based on histidine-tag chemistry.  (2012).  Anal Biochem.   421:541-6.  PMID: 22178916  doi: 10.1016/j.ab.2011.11.028.