Propargyloxy dU Modified Oligo

Propargyl dU contains an alkyne group that can be introduced to an oligonucleotide at time of synthesis using phosphoramidite chemistry. This modification can be introduced internally or at the 5' terminus. The Progargyloxy dU oligo can be conjugated with azide bearing agents and involves copper-catalyzed azide/alkyne cycloaddition reaction. Ligation of oligonucleotides containing 5' azide with 3' propargy group using click chemistry to obtain long DNA construct, has been used as PCR template. This linkage has shown to be compatible transcription and rolling circle amplification, as well as gene expression in E. coli. Long RNA containing triazole linkage has shown to retain its activity at the substrate cleavage site.

Product Information

 

Product Name:

Propargyloxy dU Modified Oligo

Category:

Click Chemistry

Modification Code:

[Propargyl dU]

Exact Mass:

784.83

Structure:

Purification:

HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

(1) Gierlich, J.; Burley, G. A.; Gramlich, P. M. E.; Hammond, D. M.; Carell, T. Org. Lett. 2006, 8, 3639-3642.
(2) Seela, F.; Sirivolu, V. R. Chem. Biodiversity 2006, 3, 509-514.
(3) Seela, F.; Sirivolu, V. R. Helv. Chim. Acta 2007, 90, 535-552.
(4) Hammond, D. M.; Manetto, A.; Gierlich, J.; Azov, V. A.; Gramlich, P. M. E.; Burley, G. A.; Maul, M.; Carell, T. Angew. Chem. Int. Ed. 2007, 46, 4184-4187.

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