Bridged Nucleic Acids (BNA)
A steady supply of large quantities of pure, bioactive proteins is critical for protein drug and assay development.
Bio-Synthesis is fully equipped to meet these needs by providing large-scale protein purification and process development to our clients, through combining our expertise in protein chemistry, analytical chemistry and functional assays. Using our versatile and reliable protein purification systems and skills, we can isolate proteins derived from natural sources, such as antibodies, enzymes, growth factors, DNA-binding proteins, membrane proteins, blood proteins or recombinant proteins expressed in different vectors..
Protein Purification Services start with the physicochemical and biological analysis of a target protein, followed by the development of tailored procedures for its extraction, purification, and characterization. Furthermore, we can assist you to modify, label and/or conjugate your protein of interest to drugs, peptides, oligonucleotides and other biomolecules.
Bio-Synthesis is committed to Total Quality Management (TQM) and standard operation procedure to assure customers' complete satisfaction. QC (quality control) and QA (quality assurance) procedures are also followed independently to offer you the guarantee of the highest quality possible for every delivered purified protein. Moreover, our dedicated technical account managers will guide you through every step of the process and constantly keep you informed of the latest project progress.
To obtain further information regarding custom bioconjugation, contact our National Customer Services Center at 800.227.0627 or contact us online for additional information or to request a quotation.
The scope of a specific protein purification plan varies depending on the target protein. Our purification strategy focuses on logically combining steps to limit the yield losses of your protein product. The protein would be initially fractionated using different extraction-partition approaches, and subsequently purified by the use of different chromatographic procedures, such as:
Our purification scale covers from milligram to gram scale. If needed, we could identify the conditions needed to prepare a lyophilized functional product.
Depending on the required needs, it is possible to renature the protein to recover its biological activity. This process could be evaluated by procedures such as difference spectra, circular dichroism spectroscopy, reactivity with monoclonal antibodies’ specific for conformation determinants and other methods.
Characterization of your product would involve different polyacrylamide gel electrophoresis under non-denaturing and denaturing conditions, amino acid composition, peptide mapping and immunological characterization. Upon request, we could do a partial amino acid sequence of the protein.
In the case of glycosylated proteins, an analysis of the carbohydrate composition can be done upon request; if required, we could isolate the different oligosaccharides for further characterization. A somewhat similar characterization may be done in the case of proteins showing post-translational modifications, like acylated proteins.
Furthermore, proteins can be labeled with a variety of dyes, functional linkers can be attached and many adducts (including but not limited to anticancer drugs) can be attached.
Although the objectives should be clearly defined prior starting of the project, we understand that new information will appear that was neither foreseen nor planed for. Nonetheless, Bio-Synthesis will try our best to purify your protein of interest while maintaining optimization of activity, purity, and yield.
For a price quote, please fill out the following form and email to us at firstname.lastname@example.org
A successful protein purification relies on optimized purification protocols. Depending on the requirements and nature of the protein, targeted purification protocols can be developed at Bio-Synthesis. We also accept purification protocols specified by our clients or obtained from the literature.
All custom purified proteins are supplied to the following: