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Protein Purification Services
Protein Purificaiton Services

A steady supply of large quantities of pure, bioactive proteins is critical for protein drug and assay development. Bio-Synthesis is fully equipped to meet these needs by providing large-scale protein purification and process development to our clients, through combining our expertise in protein chemistry, analytical chemistry and functional assays. Using our versatile and reliable protein purification systems and skills, we can isolate proteins derived from natural sources, such as antibodies, enzymes, growth factors, DNA-binding proteins, membrane proteins, blood proteins or recombinant proteins expressed in different vectors..

Protein Purification Services start with the physicochemical and biological analysis of a target protein, followed by the development of tailored procedures for its extraction, purification, and characterization. Furthermore, we can assist you to modify, label and/or conjugate your protein of interest to drugs, peptides, oligonucleotides and other biomolecules.

Bio-Synthesis is committed to Total Quality Management (TQM) and standard operation procedure to assure customers' complete satisfaction. QC (quality control) and QA (quality assurance) procedures are also followed independently to offer you the guarantee of the highest quality possible for every delivered purified protein. Moreover, our dedicated technical account managers will guide you through every step of the process and constantly keep you informed of the latest project progress.

To obtain further information regarding custom bioconjugation, contact our National Customer Services Center at 800.227.0627 or contact us online for additional information or to request a quotation.

Our Advantages

  • Free consultation
  • High success rate from various protein sources
  • Versatile protein purification system
  • Extensive quality control system
  • Dedicated columns are used for each project
  • Batch records and documentation

The scope of a specific protein purification plan varies depending on the target protein. Our purification strategy focuses on logically combining steps to limit the yield losses of your protein product. The protein would be initially fractionated using different extraction-partition approaches, and subsequently purified by the use of different chromatographic procedures, such as:

  • Ion-Exchange Chromatography – Exploits the reversible interactions between a protein and a charged chromatography medium.
  • Size Exclusion Chromatography – Separates proteins based on their differences in size.
  • Hydrophobic Interaction Chromatography – Exploits the reversible interactions between a protein and a hydrophobic ligand bound to the chromatography matrix.
  • Affinity Chromatography– Takes advantage of the reversible interactions between a protein and its specific ligand linked to a chromatography matrix.
  • Displacement Chromatography – Proteins loaded onto the chromatography matrix are displaced by a train of solutes that are more strongly bound than the loaded protein components.

Our purification scale covers from milligram to gram scale. If needed, we could identify the conditions needed to prepare a lyophilized functional product.

Depending on the required needs, it is possible to renature the protein to recover its biological activity. This process could be evaluated by procedures such as difference spectra, circular dichroism spectroscopy, reactivity with monoclonal antibodies’ specific for conformation determinants and other methods.

Characterization of your product would involve different polyacrylamide gel electrophoresis under non-denaturing and denaturing conditions, amino acid composition, peptide mapping and immunological characterization. Upon request, we could do a partial amino acid sequence of the protein.

In the case of glycosylated proteins, an analysis of the carbohydrate composition can be done upon request; if required, we could isolate the different oligosaccharides for further characterization. A somewhat similar characterization may be done in the case of proteins showing post-translational modifications, like acylated proteins.

Furthermore, proteins can be labeled with a variety of dyes, functional linkers can be attached and many adducts (including but not limited to anticancer drugs) can be attached.

Although the objectives should be clearly defined prior starting of the project, we understand that new information will appear that was neither foreseen nor planed for. Nonetheless, Bio-Synthesis will try our best to purify your protein of interest while maintaining optimization of activity, purity, and yield.

For a price quote, please fill out the following form and email to us at info@biosyn.com

Custom Protein Purification Services

A successful protein purification relies on optimized purification protocols. Depending on the requirements and nature of the protein, targeted purification protocols can be developed at Bio-Synthesis. We also accept purification protocols specified by our clients or obtained from the literature.

  • Client-supplied protocols
  • Published protocols
  • Improved protocols from client-supplied or published protocols
  • Protocols tailored to the client's requirements

All custom purified proteins are supplied to the following:

  • Specified protein concentration
  • Special formulation to prevent protein from degradation
  • Custom aliquoting as specified by customer
  • Certificate of analysis as specified by customer

Types of Protein

  • Native proteins from natural sources
  • Antibodies of different isotypes including IgMs
  • Enzymes
  • Growth factors
  • DNA binding proteins
  • Blood proteins
  • Membrane proteins
  • Fusion and tagged recombinant proteins

Protein Purification Scales

  • Analytical to preparative scale
  • Microgram to multi-gram quantities

Protein Purification Systems

  • Ion-exchange Chromatography
  • Size Exclusion Chromatography
  • Hydrophobic Interaction Chromatography
  • Affinity Chromatography (Ni, Co, FLAG, maltose, glutathione, protein A/G)
  • Displacement Chromatography

Protein Analysis

  • Amino acid analysis
  • Protein sequencing
  • Carbohydrate composition analysis
  • Electrophoresis (SDS-PAGE, native-PAGE, IEF-PAGE, urea-PAGE)
  • Antibody Isotyping
  • Immunoblot (Western Blot, dot blot)
  • Protein assay (A280, BCA, or equivalent
  • ELISA (direct or sandwich assay)
  • Contamination DNA assay
  • UV-Vis absorption spectrum
  • Endotoxin testing and analysis

Protein Characterization

  • Analytical size exclusion chromatography (native MW estimate, aggregation analysis)
  • Analytical ion-exchange chromatography
  • Purity analysis by reverse phase HPLC
  • Protein deglycosylation analysis
  • UV-Vis light absorption spectroscopy
  • Spectrofluorometric characterization
  • Analysis of oxidation, degradation and aggregation products

Additional Protein Services

  • Protein refolding
  • Protein deglycosylation
  • Peptide mapping
  • Endotoxin removal
  • Tag removal
  • Protein labeling and bioconjugation
  • Protein purification technology transfer to a GMP facility

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please call 1-972-420-8505 or Fax at 1-972-420-0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."