- Free consultation
- High success rate from various protein sources
- Versatile protein purification system
- Extensive quality control system
- Dedicated columns are used for each project
- Batch records and documentation
The scope of a specific protein purification plan varies depending on the target protein. Our purification strategy focuses on logically combining steps to limit the yield losses of your protein product. The protein would be initially fractionated using different extraction-partition approaches, and subsequently purified by the use of different chromatographic procedures, such as:
- Ion-Exchange Chromatography – Exploits the reversible interactions between a protein and a charged chromatography medium.
- Size Exclusion Chromatography – Separates proteins based on their differences in size.
- Hydrophobic Interaction Chromatography – Exploits the reversible interactions between a protein and a hydrophobic ligand bound to the chromatography matrix.
- Affinity Chromatography– Takes advantage of the reversible interactions between a protein and its specific ligand linked to a chromatography matrix.
- Displacement Chromatography – Proteins loaded onto the chromatography matrix are displaced by a train of solutes that are more strongly bound than the loaded protein components.
Our purification scale covers from milligram to gram scale. If needed, we could identify the conditions needed to prepare a lyophilized functional product.
Depending on the required needs, it is possible to renature the protein to recover its biological activity. This process could be evaluated by procedures such as difference spectra, circular dichroism spectroscopy, reactivity with monoclonal antibodies’ specific for conformation determinants and other methods.
Characterization of your product would involve different polyacrylamide gel electrophoresis under non-denaturing and denaturing conditions, amino acid composition, peptide mapping and immunological characterization. Upon request, we could do a partial amino acid sequence of the protein.
In the case of glycosylated proteins, an analysis of the carbohydrate composition can be done upon request; if required, we could isolate the different oligosaccharides for further characterization. A somewhat similar characterization may be done in the case of proteins showing post-translational modifications, like acylated proteins.
Furthermore, proteins can be labeled with a variety of dyes, functional linkers can be attached and many adducts (including but not limited to anticancer drugs) can be attached.
Although the objectives should be clearly defined prior starting of the project, we understand that new information will appear that was neither foreseen nor planed for. Nonetheless, Bio-Synthesis will try our best to purify your protein of interest while maintaining optimization of activity, purity, and yield.
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