By using a combination of an high quality synthesis and purification process developed in house and double purification provide purity beyond 95% for oligo between 8 to 150 bases.
Using the principal of molecular weight and charge group, denaturing acrylamide gel electrophoresis are used to provide higher degree of purity than HPLC (93-95%). this is recommended for purification of oligonucleotides longer than 80 bases.
Typical yields a product of 80-90% purity. The capacity and resolving properties of HPLC columns are also much greater than cartridge devices, so HPLC is the method of choice for purifying larger quantities of oligos (i.e. >=1 umol). As with cartridges, reverse-phase HPLC is usually not recommended for purifying oligos longer than 60 bases.
By using the difference in hydrophobicity between the full-length product with a 5' DMT group and truncated sequences (without DMT groups). The RP-cartridge method is typically used for oligo up to 39 bases and RP-LP cartridge use for oligonucleotide between 40 and 80 bases. Thus giving 75-85% purity for oligos up to the 1 umole scales.
At Bio-Synthesis, every oligo is desalted free of charge. Desalting removes residual by-products from the synthesis, cleavage, and deprotection procedures. Desalting is fine for oligos <=30 bases; the overwhelming abundance of full length oligo outweighs any contributions from shorter oligos.
If you have a query regarding any of our products or services, please don't hesitate to submit your question below. A member of our support team will review and answer your question as quickly as possible.
Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.