Aniline Oligonucleotide Modification

Bio-Synthesis offers aniline modificat at 5' and 3' end of an oligonucloeitde. Aniline modified oligonucleotide can function as an amino or diazo linker. Oligo modified with aniline can acts as a catalyst inthe hydrazide-aldehyde reactions. The aromatic amine of aniline rapidly and efficiently forms a Schiff base with the aldehyde, effectively increasing the activation of the aldehyde. As a result, the aniline is easily replaced by the hydrazide. Thus, aniline (also called GlycoLink Coupling Catalyst) allows significantly greater total coupling yields with hydrazides and/or greater efficiency (i.e., equal yields with less hydrazide reagent).

See the following references for additional information about aniline catalysis:

1. Byeon, J.Y., et al. (2010). Efficient bioconjugation of protein capture agents to biosensor surfaces using aniline-catalyzed hydrazone ligation.
2. Langmuir 26(19):15430-5. Dirksen, A., et al. (2006). Nucleophilic catalysis of hydrazone formation and transimination: implications for dynamic covalent chemistry. J. Am. Chem. Soc. 128(49):15602-3.
3. Dirksen, A., Dawson, PE. (2008). Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling. Bioconjug. Chem. 19(12):2543-8.

Product Information

 

Product Name:

Aniline Oligonucleotide Modification

Category:

linker, amino linker, diazo-linker

Modification Code:

[Aniline]

Chemical Formula:

C7H6NO

Exact Mass:

120.04

Formula Weight:

120.13

m/z:

120.04 (100.0%), 121.05 (7.7%)

Elemental Analysis:

C 69.99; H, 5.03; N, 11.66; O, 13.32

Structure:

Purification:

HPLC or PAGE

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

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5' Adenylation Oligonucleotide Modification

Maleimide

Aldehyde Oligo Modification

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