Bio-Synthesis provides tree-like branching DNA or RNA synthesis by incorporating a long trebler at 5' end of an oligonucleotide using standard oligonucleotide synthesis chemistry. Dendrimers with a positively charged surfaces interact strongly with nucleic acids, helping to transport them through the membranes of cells1; dendrimers with internal cavities interact non-covalently with guest molecules to give guest-host systems2. In oligonucleotide chemistry, branched oligonucleotides4 were used for signal amplification in hybridization tests, making it possible to detect the target at levels under 100 molecules/mL3.  Fluorescent signal also increases in porportion to the number of 5' ends. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease, making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the 'bottom up' approach to nano-assembly. These features also apply to DNA chip technology when higher temperatures are required, to melt a secondary structure in the target.

Contact us for long trebler branch oligonucleotide synthesis.

Product Information


Product Name:

Long Trebler Branch Oligo Synthesis


Dendrimer; Branch Synthesis


Bio-Synthesis Inc. Oligo Structure



Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.


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