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Photocleavable Oligonucleotide

Bio-Synthesis offers several types of photocleavable oligonucleotide modifiers to be introduced at 5', 3' position or internally during chemical synthesis. Photo-triggered oligonucleotide is a major tool used for studying conformational changes and strand breaks, as well as for studying activation of nucleic acid targeted drug, such as antisense oligonucleotide1. The PC-oligonucleotides can be immobilized on a solid support or specifically labeled with a hapten, fluorophore or other detectable group. The oligonucleotide can then be detached from the support or the label removed by brief exposure to UV-light.

As one of the pioneers providing chemical biology products, Bio-Synthesis is committed to develop new technologies. Contact Bio-Synthesis for Photocleavable Oligonucleotide Synthesis Services.

Photo Cleavable Oligonucleotide Modification

We offer several photocleavable modifiers that are suitable for following applications.

  • 5' PC biotinylated oligonucleotide exhibit similar properties to 5' biotin modification. They are suitable for capture by streptavidin. PC Biotin is rapidly and quantitatively cleaved from the 5'-terminus of the oligonucleotide using near-UV light at 300 þ 350nm.

  • PC amino or thiol modified oligonucleotides have proven to be very useful for the attachment of a variety of haptens and fluorophores, as well as for the tethering of the oligonucleotides to a diversity of beads and surfaces. PC amino modifier can be modified at 5' end of oligonucleotides that are suitable for subsequence photocleavage.

  • PC spacer can be used as an intermediary to attach any modification to the terminal end of oligonucleotides. After cleavage, a 5'-phosphate is generated on the DNA or RNA, rendering it suitable for further biological transformations, such as gene construction and cloning after ligation.

Product Code Catalog No. 5' Internal 3' DNA RNA
PC Amino C6(Photocleavable) [PCAmC6] PCO1001 Y N N Y Y
PC Biotin (Photocleavable) [PCBio] PCO1002 Y N Y Y Y
PC Linker (Photocleavable) [PCL] PCO1003 Y N Y Y Y
PC Spacer (Photocleavable) [PCSPC] PCO1004 Y N Y Y Y

Purification

Desalting or cartridge purification is acceptable for spacer modified oligonucleotide. However, additional purification by HPLC is strongly recommended.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate .

Cleavage Protocol

Optimal cleavage is obtained with exposure to long-wave UV light in the 300-350 nm spectral range. Cleavage releases the oligo with a 5'-phosphate group.

Reference:

1. P. Ordoukhanian and J-S. Taylor, J. Am. Chem. Soc., 117, 9570-9571, 1995.
2. (a) F. Hausch and A. Jäschke, Nucleic Acids Research, 2000, 28, e35.
(b) F. Hausch and A. Jäschke, Tetrahedron, 2001, 57, 1261-1268.
3. T. Wenzel, T. Elssner, K. Fahr, J. Bimmler, S. Richter, I. Thomas, and M. Kostrzewa, Nucleosides, Nucleotides & Nucleic Acids, 2003, 22, 1579-1581.

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  • Please contact us for additional information or send an email to info@biosyn.com.
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