2' Fluoro RNA oligonucleotides contain a fluorine molecule at the 2' ribose position (instead of a 2'-hydroxyl group in an RNA monomer). The main benefit is increased binding affinity (Tm) and nuclease resistance. Fluoro RNA and standard RNA bases share similar properties which allow 2'-F RNA to form stable duplexes with RNA targets. Furthermore, the stability of 2' Fluro RNA-RNA duplexes is higher than normal RNA-RNA duplexes by about 1-2 degrees-C per each substitution.
Replacement of 2'-hydroxyl group with 2'-fluoro has also shown to be more stable for chemical hydrolysis at high pH as compared with internucleotide linkages in unmodified RNA oligonucleotides.
2' fluoro modified bases can be purified using standard DNA purification methods. However, yields may vary depending on the oligonucleotide design.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.