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Bio-Synthesis offers a wide variety of backbone modified oligonucleotide synthesis to increase oligonucleotide affinity to its complementary strands which often results in drastic improvement in specificity and sensitivity for applications where detection of small or highly similar DNA or RNA targets. These backbone and linkage modified oligonucleotides not only possess strong affinity-enhancing effects but also increase resistance to endo and exonucleases which leads to high in vitro and in vivo stability.

We offer the following modifications:

Contact Bio-Synthesis for Backbone Modified Oligonucleotide Synthesis Services.

Our backbone modified base oligo synthesis service offers you with:

  • Scale: 100 nmol, 250 nmole, 1 umole or greater.
  • Purification options: Desalted, PAGE, HPLC, dual HPLC
  • Oligo lengths: 5-100 bases
  • TAT: 2 - 4 working days
  • Delivery: lyophilized in tube format or plate
  • Concentration adjustment and additional aliquoting available
  • Quality control by OD measurement and MALDI-TOF MS
Product Nuclease Resistance RNase H Activation Chimeric Function
Bridged Nucleic Acid (BNA) Yes Yes Yes
  • The ability to increase base-discrimination
  • Increase stability of duplex or triplex formation
  • Strong nuclease resistence
2' Fluoro RNA Yes No Yes
  • Increase thermo stability with RNA
  • Increase nuclease resistance
2'-F-ANA Yes Yes Yes
  • Increase thermo stability with DNA
2' O-Methyl RNA (2'OMe) Yes No Yes
  • Greater stability than DNA and RNA hybrids
  • Resistance to a wide variety of ribo and deoxyribonuclease
L-DNA Yes No Yes
  • A left-helical double-helix
  • Immune to nuclease attack
L-RNA Yes No Yes
  • A left-helical double-helix
  • Immune to nuclease attack
Phosphonoacetate (PACE) Yes Yes Yes
  • Enhance penetration of cultured cells
ZNA Spermine No No Yes
  • Stabilizing the formed duplex by reducing electrostatic repulsion
  • Enhance cell delivery
  • Improved quenching, an alternative to Minor Groove Binder (MGB)
Phosphorothioate DNA Yes Yes Yes
  • Increased cell uptake
  • Nuclease resistance
  • Elicitation of RNAse H activity
Phosphorothioate RNA yes yes Yes
  • Increased cell uptake
  • Nuclease resistance
  • Elicitation of RNAse H activity
Methylphosphonate Yes No Yes
  • Introduce a neutral backbone
  • High stability to nuclease degradation

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please call 1-972-420-8505 or Fax at 1-972-420-0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."