Oligonucleotides containing 3-cyanovinylcarbazole (CNVK) can be rapidly cross-linked to a complementary strand at UV 366nm without causing significant DNA damage1. In addition, studies have shown that purine bases were unreactive to cross-linking, allowing differentiation between pyrimidines and purines at the target site. Once cross-linked, the UV melting temperature of the duplex was raised by around 30°C (relative to the duplex temperature before irradiation. Complete reversal of the cross-link takes place at 312nm in 3 approximately minutes.

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Product Information


Product Name:

3-cyanovinylcarbazole nucleoside CNVK Ultrafast Photo Cross-Linker


photo cross-linker

Modification Code:



Bio-Synthesis Inc. Oligo Structure



Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.


1. Y. Yoshimura, and K. Fujimoto, Org Lett, 2008, 10, 3227-30.
2. K. Fujimoto, K. Konishi-Hiratsuka, T. Sakamoto, and Y. Yoshimura, ChemBioChem, 2010, 11, 1661-4.
3. Y. Yoshimura, T. Ohtake, H. Okada, and K. Fujimoto, ChemBioChem, 2009, 10, 1473-6.