Oligonucleotides containing 3-cyanovinylcarbazole (CNVK) can be rapidly cross-linked to a complementary strand at UV 366nm without causing significant DNA damage1. In addition, studies have shown that purine bases were unreactive to cross-linking, allowing differentiation between pyrimidines and purines at the target site. Once cross-linked, the UV melting temperature of the duplex was raised by around 30°C (relative to the duplex temperature before irradiation. Complete reversal of the cross-link takes place at 312nm in 3 approximately minutes. Contact us for 3-cyanovinylcarbazole (CNVK) Modified Oligonucleotide Synthesis
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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1. Y. Yoshimura, and K. Fujimoto, Org Lett, 2008, 10, 3227-30. 2. K. Fujimoto, K. Konishi-Hiratsuka, T. Sakamoto, and Y. Yoshimura, ChemBioChem, 2010, 11, 1661-4. 3. Y. Yoshimura, T. Ohtake, H. Okada, and K. Fujimoto, ChemBioChem, 2009, 10, 1473-6.