p300 and p300/cAMP-response Element-binding Protein-associated Factor Acetylate the Androgen Receptor at Sites Governing Hormone-dependent Transactivation
Maofu Fu, Chenguang Wang, Anne T. Reutens, Jian Wang, Ruth H. Angeletti, Linda Siconolfi-Baez, Vasily Ogryzko, Maria-Laura Avantaggiati, and Richard G. Pestelli
01/15/2013
Vol. 275, No. 27, Issue of July 7, pp. 20853–20860, 2000
The androgen receptor (AR) is a sequence-specific DNA-binding protein that plays a key role in prostate cancer cellular proliferation by dihydrotestosterone and the induction of secondary sexual characteristics. In this study we demonstrate that the AR can be modified by acetylation in vitro and in vivo. p300 and p300/ cAMP-response element-binding protein acetylated the AR at a highly conserved lysine-rich motif carboxylterminal to the zinc finger DNA-binding domain. [14C]acetate-labeling experiments demonstrated that AR acetylation by p300 in cultured cells requires the same residues identified in vitro. Point mutation of the AR acetylation site (K632A/K633A) abrogated dihydrotestosterone-dependent transactivation of the AR in cultured cells. Mutation of the p300 CH3 region or the p300/cAMP-response element-binding protein histone acetylase domain reduced ligand-dependent AR function. The identification of the AR as a direct target of histone acetyltransferase o-activators has important implications for targeting inhibitors of AR function.