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How to design a TaqMan Assay

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How to design a TaqMan Assay

In RNA quantitation assays the TaqMan Universal PCR Master Mix is used in the second step of a two-step reverse transcription–polymerase chain reaction (RT-PCR) protocol. The template is cDNA generated from a reverse transcription reaction.

The TaqMan Universal PCR Master Mix may be used for real-time or plate read (endpoint) detection of DNA or cDNA.

Click on PDF link for details.

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How to design a TaqMan Assay

How to design a TaqMan Assay

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