Semaphorin 3B Is a 1,25-Dihydroxyvitamin D3-Induced Gene in Osteoblasts that Promotes Osteoclastogenesis and Induces Osteopenia in Mice

Amelia L. M. Sutton,* Xiaoxue Zhang,* Diane R. Dowd, Yogendra P. Kharode, Barry S. Komm, and Paul N. MacDonald

3[1,25(OH)intestinal absorption of calcium and phosphate and directly by acting on osteoblasts and osteoclasts. Despite the direct actions of 1,25(OH) 2D3] impacts bone indirectly by promoting 2D3 in bone, relatively little is known of the mechanisms or target genes that are regulated by 1,25(OH) semaphorin 3B (SEMA3B) as a 1,25(OH) gene in osteoblastic cells. Northern analysis revealed strong induction of SEMA3B mRNA by 1,25(OH)osteoblastic cells. Moreover, differentiation of these osteogenic cells enhanced SEMA3B gene expression. Biological effects of SEMA3B in the skeletal system have not been reported. Here, we show that osteoblast-derived SEMA3B alters global skeletal homeostasis in intact animals and osteoblast function in cell culture. Osteoblast-targeted expression of SEMA3B in mice resulted in reduced bone mineral density and aberrant trabecular structure compared with nontransgenic littermates. Histomorphometry studies indicated that this was likely due to increased osteoclast numbers and activity. Indeed, primary osteoblasts obtained from SEMA3B transgenic mice stimulated osteoclastogenesis to a greater extent than nontransgenic osteoblasts. This study establishes that SEMA3B is a 1,25(OH) and that osteoblast-derived SEMA3B impacts skeletal biology these studies support a putative role for SEMA3B as an osteoblast protein that regulates bone mass and skeletal homeostasis. ( Endocrinology 2D3 in skeletal cells. Here, we identify 2D3-stimulated 2D3 in MG-63, ST-2, MC3T3, and primary 2D3-induced gene in osteoblasts in vitro and in vivo.Collectively, Molecular 22: 1370–1381, 2008)

The vitamin D endocrine system is important for skeletal homeostasis. 1,25-Dihydroxyvitamin D