Bio-Synthesis offers C3 Spacer oligonucleotide modification at various scales. The C3 propyl spacer can be incorporated internally or at the either end of the oligo during chemical synthesis. Multiple C3 spacers can be added to introduce a longer spacer arm for the attachment of fluorophores or other pendent groups.

Spacer C3-modified oligos have been used in a number of different applications, including protein-RNA functional studies1 , as a DNA abasic site mimic to study the utility of small synthetic ligands (such as pteridines) for nucleotide recognition in SNP typing applications2, and for solid-phase immobilization of hybridization probes3 . Spacer C3 incorporated at the 3’-end of an oligo functions as an effective blocking agent against polymerase extension at that end in PCR reactions4

Contact Bio-Synthesis for C3 Spacer Oligonucleotide Modification Service.

Product Information


Product Name:

C3 Spacer, Spacer C3 Oligonucleotide Modification

Alternate Name:

Spacer C3


spacer, terminator, blocking agent

Modification Code:


Chemical Formula:


Exact Mass:


Formula Weight:



138.01 (100.0%), 139.01 (3.5%)

Elemental Analysis:

C, 26.10; H, 5.11;O,46.35; P, 22.44


Bio-Synthesis Inc. Oligo Structure



Delivery Format:


Shipping Conditions:

Room temperature

Storage Conditions:

-20 °C to -70 °C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.


  1. Pritchard, C.E., Grasby, J.A., Harny, F., Zacharek, A.M., Singh, M., Karn, J., Gait, M.J. Methylphosphonate mapping of phosphate contacts critical for RNA recognition by the human immunodeficiency virus tat and rev proteins. Nucleic Acids Res. (1994), 22: 2592-2600.
  2. Dai, Q., Xu, C-Y., Sato, Y., Yoshimoto, K., Nishizawa, S., Teramae, N. Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group. Anal. Sci. (Japan) (2006), 22: 201-203.
  3. Li, H., McGall, G. Photoactivatable Silanes: Synthesis and Uses in Biopolymer Array Fabrication on Glass Substrates. In Frontiers in Biochip Technology, X, W-L., Cheng, J. (Ed.). Springer Science+Business Media, Inc. (2006). pp. 176-190.
  4. Zhou, L., Myers, A.N., Vandersteen, J.G., Wang, L, Wittwer, C.T. Closed-Tube Genotyping with Unlabeled Oligonucleotide Probes and a Saturating DNA Dye. Clin. Chem. (2004), 50: 1328-1335.