Spacer 9, spacer 18, Spacer C12, Spacer C3 can be inserted in multiple location within can oligonucleotide when a longer spacer is required. Spacer 9 is a triethylene glycol chain that is 9 atoms long (6 carbons + 3 oxygens). Spacer 9 has been used in various applications example such as using spacer 9 to form non-nucleotide bridges in hairpin loops in oligonucleotides Spacer 9 modified oligonucleotides can be used for epitope drug developmen. Other application such as solid-phase immobilization of hybridization probes. Multiple addition of Spacer 9 has been used to form long, flexible linker arms between the two domains of a bifunctional DNA oligonucleotide, in order to maximize the binding flexibility.

Contact Bio-Synthesis for Spacer 9 Modified Oligonucleotide Synthesis.

Product Information


Product Name:

Spacer 9 Oligonucleotide Modification


spacer, linker, PEG linker, TEG

Modification Code:


Chemical Formula:


Exact Mass:


Formula Weight:



212.04 (100.0%), 213.05 (6.9%), 214.05 (14%)

Elemental Analysis:

C, 33.97; H,6,18;O,45.25; P,14.60


Bio-Synthesis Inc. Oligo Structure



Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.


  1. Nelson, J.S., Giver, L., Ellington, A.D., Letsinger, R.L. Incorporation of Non-Nucleotide Bridge into Hairpin Oligonucleotides Capable of High-Affinity Binding to the Rev Protein of HIV-1. Biochemistry. (1996), 35: 5339-5344.
  2. Palma, E., Klapper, D.G., Cho, M.J. Antibodies as Drug Carriers III: Design of Oligonucleotides with Enhanced Binding Affinity for Immunoglobulin G. Pharm. Res. (2005), 22: 122-127.
  3. Beattie, W.G., Meng, L., Turner, S.L., Varma, R.S., Dao, D.D., Beattie, K.L. Hybridization of DNA targets to glass-tethered oligonucleotide probes. Mol. Biotechnol. (1995), 4: 213-225.
  4. Rogers, F.A., Vasquez, K.M., Egholm, M., Glazer, P.M. Site-directed recombination via bifunctional PNA-DNA conjugates. Proc. Natl. Acad. Sci. USA (2002), 99: 16695-16700. - Spacer 9