The polymerase chain reaction (PCR) technology is one of the essential methods used in molecular biology to detect DNA and RNA sequences. Single copies of specific oligonucleotide sequences can be amplified and detected.
There is a quantitative response to starting material and the amount of PCR product at any amplification cycle. However, the yields in PCR products can vary in replicate reactions.
Real-time quantitative PCR eliminated variabilities traditionally associated with quantitative PCR. As a result, routine and reliable quantitation of PCR products is now possible.