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Can BNA be incorporated in DNA primers?

BNA can be mixed with DNA, RNA and other nucleic acid analogs within the oligonucleotide to facilitate changes of Tm (melting temperature) without losing specificity. They allow for shorter probe design while maintaining the same Tm. The Tm of a nucleotide duplex can be modulated by varying the BNA content. This feature can be used to normalize the Tm across a population of short sequences with varying GC-content for AT-rich nucleotides with low melting temperatures.