Antibodies can deliver ASOs specifically to cells expressing a particular surface marker for example on cancer cells, immune cells, or hepatocytes, useful for targeted drug delivery. Many cell types cannot naturally take up ASOs well. However, antibody-mediated delivery can enhance cellular internalization allowing improved uptake of conjugates. Targeted delivery reduces systemic distribution and toxicity thereby reducing off-target effects.
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"BioSynthesis provides a full spectrum of high quality custom antisense oligonucleotide synthesis as well as biotinylated and capped RNA including capped mRNA.
BioSynthesis also provides the synthesis of long and circular oligonucleotides, including services of back-bone modifications, conjugation to antibodies, peptides, proteins and fatty acids.
Biotinylation is possible by direct solid-phase chemical synthesis or enzyme-assisted approaches to obtain artificially modified oligonucleotides, such as BNA/LNA antisense oligonucleotides, mRNAs or siRNAs, containing a natural or modified backbone, base, sugar and internucleotide linkages.
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AOCs: antibody-bioconjugation
5'-capped oligonucleotides:
ARCA What-is-ARCA?
Capped oligonucleotides
5'-capped-oligonucleotides
Guanosine-triphosphate-oligo-capping
The-5-Guanosine-triphosphate-Cap
N7-methylguanosine-triphosphate-terminal-cap
mRNA-Capping-and-Decapping-Enzymes
https://www.biosyn.com/tew/Function-and-Application-of-5%E2%80%99-Triphosphate-Oligonucleotides.aspx
https://www.biosyn.com/tew/SARS-CoV-2-modifies-the-host-s-mRNA-cap-to-evade-an-innate-immunity-response!.aspx
Guanosine-triphosphate-oligo-capping
Thiamine-(Vitamin-B1)-and-RNA-capping
The-RNA-TrimethylGuanosine-or-TMG-cap
n7-methylguanosine-triphosphate-mcap
n7-methylguanosine-triphosphate-terminal-cap.aspx
RNA-Capping-and-De-Capping
Analysis
Mass-spectrometry-combined-with-X-ray-crystallography-allows-the-characterization-of-cap-protein-interactions
RNA-cap-epitranscriptome-analysis-reveals-novel-caps
The-efficacy-of-fda-approved-drug-inhibiting-the-cap-snatching-endonuclease-of-rdrp-polymerase-is-undermined-by-the-emergence-of-resistant-influenza-virus-strains
Aptamer
Effect-of-3-end-capping-of-aptamer-with-various-2-,4-bridged-nucleotides,-Enzymatic-post-modification-toward-a-practical-use-of-polyclonal-aptamers
Production
Large-scale-production-of-capped-RNA-as-a-service
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