800.227.0627

What are the reasons for conjugating antibodies to ASOs?

Antibodies can deliver ASOs specifically to cells expressing a particular surface marker for example on cancer cells, immune cells, or hepatocytes, useful for targeted drug delivery. Many cell types cannot naturally take up ASOs well. However, antibody-mediated delivery can enhance cellular internalization allowing improved uptake of conjugates. Targeted delivery reduces systemic distribution and toxicity thereby reducing off-target effects.

---...---

"BioSynthesis provides a full spectrum of high quality custom antisense oligonucleotide synthesis as well as biotinylated and capped RNA including capped mRNA.

BioSynthesis also provides the synthesis of long and circular oligonucleotides, including services of back-bone modifications, conjugation to antibodies, peptides, proteins and fatty acids.

Biotinylation is possible by direct solid-phase chemical synthesis or enzyme-assisted approaches to obtain artificially modified oligonucleotides, such as BNA/LNA antisense oligonucleotides, mRNAs or siRNAs, containing a natural or modified backbone, base, sugar and internucleotide linkages.

---...---

AOCs: antibody-bioconjugation

5'-capped oligonucleotides:

ARCA What-is-ARCA?

Capped oligonucleotides 

5'-capped-oligonucleotides

Guanosine-triphosphate-oligo-capping

The-5-Guanosine-triphosphate-Cap

N7-methylguanosine-triphosphate-terminal-cap

mRNA-Capping-and-Decapping-Enzymes

https://www.biosyn.com/tew/Function-and-Application-of-5%E2%80%99-Triphosphate-Oligonucleotides.aspx

https://www.biosyn.com/tew/SARS-CoV-2-modifies-the-host-s-mRNA-cap-to-evade-an-innate-immunity-response!.aspx

Guanosine-triphosphate-oligo-capping

Thiamine-(Vitamin-B1)-and-RNA-capping

The-RNA-TrimethylGuanosine-or-TMG-cap

n7-methylguanosine-triphosphate-mcap

n7-methylguanosine-triphosphate-terminal-cap.aspx

RNA-Capping-and-De-Capping

Analysis

Mass-spectrometry-combined-with-X-ray-crystallography-allows-the-characterization-of-cap-protein-interactions

RNA-cap-epitranscriptome-analysis-reveals-novel-caps

The-efficacy-of-fda-approved-drug-inhibiting-the-cap-snatching-endonuclease-of-rdrp-polymerase-is-undermined-by-the-emergence-of-resistant-influenza-virus-strains

Aptamer

Effect-of-3-end-capping-of-aptamer-with-various-2-,4-bridged-nucleotides,-Enzymatic-post-modification-toward-a-practical-use-of-polyclonal-aptamers

Production

Large-scale-production-of-capped-RNA-as-a-service

---...---