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What should we consider for the conjugation of antibodies to antisense oligonucleotides?

Considerations are:

Site-specific versus random conjugation: Site-specific methods utilizing cysteines or unnatural amino acids preserve antibody interactions.

Linker stability: The choice of linkers affects circulation time and intracellular release.

Preserving ASO activity: Ensure the selected conjugation approach does not hinder ASO hybridization or RNase H recruitment, if applicable.

Scale and purification: Ab-ASO conjugates can be complex to purify and characterize.

Bioconjugation and Linkers

Peptide-Linkers-and-Linker-Peptides-for-Antibody-Drug-Conjugates-(ADCs)

Photocleavable-oligonucleotide-synthesis

Photocleavable-pc-biotin-modified-oligos

Bioconjugation

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