Considerations are:
Site-specific versus random conjugation: Site-specific methods utilizing cysteines or unnatural amino acids preserve antibody interactions.
Linker stability: The choice of linkers affects circulation time and intracellular release.
Preserving ASO activity: Ensure the selected conjugation approach does not hinder ASO hybridization or RNase H recruitment, if applicable.
Scale and purification: Ab-ASO conjugates can be complex to purify and characterize.
Bioconjugation and Linkers
Peptide-Linkers-and-Linker-Peptides-for-Antibody-Drug-Conjugates-(ADCs)
Photocleavable-oligonucleotide-synthesis
Photocleavable-pc-biotin-modified-oligos
Bioconjugation
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