Chemical RNA synthesis vs RNA Synthesis by Transcription
long RNA Transcription synthesis is done by using an RNA polymerase to produces many copies of RNA by transcribing a DNA template which encodes the RNA. RNA synthesis by transcription is an alternative to chemical RNA synthesis which is extremely expensive and limited in length (up to 150 mers). Yields of chemically-synthesized RNA decrease exponentially with increasing length because coupling efficiencies at each step are 90-99% (depending on the type of incorporated base). Thus it is not feasible to synthesize very long RNA chemically especially in this case. In contrast, RNA Synthesis using bacteriophage polymerases, such as T7 RNA polymerase, are quite processive and it is therefore possible to obtain RNA by transcription that are thousands of bases long and migrate as a single band on a denaturing gel.
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08/02/2013