Enhanced Diagnostic Tools
The surest way to guarantee blocking the 3'-terminus is using a 2',3'-dideoxynucleoside support. Unfortunately, only ddC are amenable to attachment to the support through the exocyclic amino group.
In situations where it is necessary to have a selection of all four bases available, it is possible to use the 3'-deoxynucleoside supports as 3'-terminators. Although the 2'-hydroxyl group is still present in the final oligonucleotides, it is not a substrate for at least the routinely used polymerases. All four 3'-deoxynucleoside supports will shortly be available, along with their phosphoramidite counterparts.
If you have a query regarding any of our products or services,
please don't hesitate to submit your question below. A member of our support team
will review and answer your question as quickly as possible.