Enhanced Diagnostic Tools
Yes. We offer long RNA synthesis. There are two different ways we can make Long RNA for you.
We can synthesize Long RNA chemically or enzymatically (by IVT). Chemical ligation methods ensure that the exact sequence requested is correctly synthesized. Many modifications can also be incorporated into oligonucleotides. In addition, different labeling strategies are available. Using chemical synthesis methods, it is possible to synthesize larger oligonucleotides in sizes up to 200 bases long. However, since chemical methods have lower yields, the overall cost is higher than for alternative methods.
The Enzymatic method (IVT) allows the generation of very long oligonucleotide sequences (up to 3,000 and higher) at a much higher yield. The difference of this method is that oligonucleotide sequences can have three added nucleotide bases. This leader sequenceresults from the use of a promoter. Long RNA synthesis is done using bacteriophage polymerases such as T7, T3 or SP6. Depending on the complexity and length of the sequence, if the RNA is synthesized using polymerase T7 and T3, an additional GGG is added. SP6 can add an additional GAA to the oligonuclotide as a result of the presence of the promoter sequence. However, for most molecular biology applications, three extra bases on the 5' end should not interfere with downstream experiments, considering that the entire RNA oligonucleotide is no much longer.
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