How does purity of synthetic siRNA’s affect RNAi?
RNAi is a sequence specific chain of events. Chemical synthesis of siRNA’s is based on coupling of bases to yield a particular sequence. The yield and purity depends on the coupling efficiency. BioSynthesis siRNA’s can be used without further purification, but BioSynthesis recommends purified siRNA’s for use in transfection.
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04/30/2008