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Biosynthesis Sirna’s Chemical Synthesis

How does purity of synthetic siRNA’s affect RNAi?

RNAi is a sequence specific chain of events. Chemical synthesis of siRNA’s is based on coupling of bases to yield a particular sequence. The yield and purity depends on the coupling efficiency. BioSynthesis siRNA’s can be used without further purification, but BioSynthesis recommends purified siRNA’s for use in transfection.

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