Enhanced Diagnostic Tools
Quality control is essential part of any oligonucleotides synthesis process. Routinely we perform biological activity check by running oligos in PCR reactions. All reagents used in BSI laboratory comes from reliable suppliers, and each lot is extensively QC checked prior use.
To control the coupling efficiency, online trityl monitoring of all oligonucleotides were carefully checked each cycle. After synthesis, all oligonucleotides quantity are analyzed by optical density (OD260) measurement. Randomly selected unmodified and all modified oligonucleotides are identified by MALDI-TOF mass Spectrometry, and purity check by PAGE or HPLC.
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