Test for Glutamine in animal serum

1.  An aliquot of the serum will need to incubated with an aliquot of sodium borate buffer over night at pH 8.3 to dissolve the glutamine quantitatively.
2. Sulphosalicylic acid will need to be added to keep the amino acids stable
3. The proteins in the serum will need to be separated, e.g. by ethanol precipitation or a similar precipitation
4. The glutamine in the supernatant will be analyzed
5. An internal standard such as norleucine will need to be used to spike the sample for normalization of the final data
6. We most likely will need to outsource the amino acid analysis to perform a ninhydrin based analysis
7. We can do the extraction

Quantity needed: 1 to 5 ml of serum.
Price: $375/sample