Enhanced Diagnostic Tools
The protecting groups utilized during the chemical assembly of synthetic RNA are removed during the ammonium based deprotection and purification steps and analogous to GMP, a residual solvent analysis is useful. Whereas toxic heavy metals are not used during the synthetic steps, their presence is minimized by using EDTA in the HPLC buffers (EDTA is a good chelator and will remove any heavy metal that may be present). The RNA’s are obtained as the ammonium salts after synthesis and is desirable to convert them to the sodium form, by using a sodium chloride gradient in the presence of sodium phosphate buffer. Nonetheless the main concern one has to deal with is the presence of endotoxins, which when present in small amounts can cause severe inflammation and even death in laboratory animals.
Bio-Synthesis offer several purification process, and extensive QC package to minimize the potential contaminants. Contact our support at biosyn@biosyn.com
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