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Multiple antigenic peptide - MAP peptides

MAPS or Multi-Antigenic Peptide is a branched peptide at which linear peptide chains are linked at their C-terminus via polylysine core, thereby increasing the size of whole molecule. This is done to eliminate the coupling of peptides to KLH. It seems that, however, conformation of peptides on MAP is less flexible, and antibodies obtained by MAP often recognize protein less often than by conventional KLH conjugation. In addition, there is no free peptide produced when making MAP, making it difficult to remove polylysine core directed antibodies. Purification of MAP by HPLC is difficult, and MAP is provided without mass verification due do its heterogeneity and large molecular size.

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