Acidic Guanidinium Thiocynate Phenol Extraction?
Acidic Guanidinium Thiocynate Phenol Extraction: Extraction with acidic guanidinium thiocynate phenol preferentially extracts RNA while removing most proteins and DNA. A subsequent isopropanol precipitation and 70% ethanol wash concentrates the RNA and removes most protein, DNA and NTPs. For applications that are very sensitive to DNA contamination, a DNase treatment or additional purification is
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08/02/2013