What is the difference between synthesis scale and yield?
What is the difference between scale and yield?
The terminology of synthesis scale (or starting scale) often caused confusion. Synthesis scale are based on the amount of first base, which is present at the start of synthesis, i.e., 100 nmole scale means that there are 100 nmoles of base attached to the solid support. For larger scales, the quantity of solid support used during synthesis is increased. Synthesis scale does not refer to amount expected amount. Because of the inefficiency of oligonucleotide synthesis and purification, the quantity of oligonucleotide received is usually less than the scale of the theoretical amount. I t is easier to specify quantity of final yield by OD, nmole or mass. Example if you ask for 1 OD synthesis, you will receive a minimum of 1 OD260 unit. or you can ask to receive final amount by nmole or in mass units.
Scale
- Relative amount of starting material packed into an RNA synthesis column
- Typically expressed as micromole (µmol) increments
Yield
- Final amount of material delivered
- Typically expressed as nanomole (nmol), micromole (µmol), milligram (mg), or gram (g) amounts
SCALE ≠ YIELD Actual yields obtained for the final product may range from 10%-90% of the scale, depending on the oligo specifications (i.e. modification type, length, purification process, etc.).
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11/03/2011