Which quencher should I use?
Dye Name |
Ex |
Em |
Color |
BHQ based on J (λ) |
other dark quenchers: |
Alexa 350 |
346 |
442 |
Blue |
BHQ-0
λ ( max ) = 495 nm
Range = 430-520 nm |
DABCYL
λ ( max ) = 479 nm |
|
QSY-35
λ ( max ) = 475 nm |
Pacific Blue |
416 |
451 |
Marina Blue |
362 |
459 |
Blue-Green |
Eclipse
λ ( max ) = 522 nm |
Acridine |
362 |
462 |
Edans |
336 |
468 |
Green |
Coumarin |
432 |
472 |
BODIPY 493/503 |
493 |
503 |
Cy2 |
489 |
506 |
BODIPY FL-X |
504 |
510 |
Yellow-Green |
|
|
DANSYL |
335 |
518 |
Alexa 488 |
495 |
519 |
BHQ-1
λ ( max ) = 534 nm
Range = 480-580 nm |
FAM |
495 |
520 |
Oregon Green |
500 |
520 |
Rhodamine Green-X |
503 |
528 |
NBD-X |
466 |
535 |
TET |
521 |
536 |
Alexa 430 |
434 |
541 |
QSY-7&9
λ ( max ) = 560 nm |
BODIPY R6G-X |
529 |
547 |
Yellow |
JOE |
520 |
548 |
|
Yakima Yellow |
531 |
549 |
Alexa 532 |
532 |
554 |
VIC |
538 |
554 |
HEX |
535 |
556 |
R6G |
524 |
557 |
BHQ-2
λ ( max ) = 579 nm
Range = 550-650 nm |
Alexa 555 |
555 |
565 |
Yellow-Orange |
BODIPY 564/570 |
563 |
569 |
BODIPY TMR-X |
544 |
570 |
Cy3 |
550 |
570 |
Alexa 546 |
556 |
573 |
TAMRA |
555 |
576 |
Rhodamine Red-X |
560 |
580 |
BODIPY 581/591 |
581 |
591 |
|
Redmond Red |
579 |
595 |
Cy3.5 |
581 |
596 |
ROX |
575 |
602 |
Orange |
Alexa 568 |
578 |
603 |
Cal Red |
583 |
603 |
BODIPY TR-X |
588 |
616 |
Orange-Red |
Alexa 594 |
590 |
617 |
BODIPY 630/650-X |
625 |
640 |
Red |
QSY-21
λ ( max ) = 661 nm |
LC Red 640 |
625 |
640 |
BHQ-3
λ ( max ) = 680 nm
Range = 620-730 nm |
Alexa 633 |
632 |
647 |
BODIPY 650/665-X |
646 |
660 |
Alexa 647 |
650 |
665 |
Cy5 |
649 |
670 |
Alexa 660 |
663 |
690 |
Far Red |
Cy5.5 |
675 |
694 |
|
Alexa 680 |
679 |
702 |
LC Red 705 |
689 |
705 |
Alexa 700 |
702 |
723 |
Alexa 750 |
749 |
775 |
|
Note: BHQs are available as phosphoramidites and CPGs, DABCYL and Eclipse are available as phosphoramidites, CPGs and succinimidyl esters, and QSYs are available as succinimidyl esters only. |
Print
06/20/2010