After the transfection of a cell with siRNAs, their subcellular localization will need to be monitored. siRNAs labeled with fluorescent dyes allow tracking the delivery and uptake of siRNA. Usually, after uptake, the siRNA is present as a free molecule in the cytoplasm and complexed with proteins in the endosomes.
A variety of modifications is available for siRNA, such as 2’-O methyl RNA, LNA, biotin, or digoxigenin based on a researcher's need. All these modifications are available from BioSynthesis.